A Rapid Determination of ATP in Fish Muscle Using Luciferase of American Firefly Photinus pyrarlis.

Determination of ATP with a Firefly Luciferase-Based System for the Detection of Life in Mars

Equilibrium intermediates in the reversible unfolding of firefly (Photinus pyralis) luciferase.

Firefly luciferase has been used as a model protein to study cotranslational and chaperone-assisted protein folding. We found conditions for reversible unfolding of luciferase in the absence of cellular factors, and we characterized denaturant-induced equilibrium unfolding transitions and refolding kinetics of the enzyme. Luciferase unfolding induced by guanidinium chloride at 10 degrees C can ...

Regulatory effects of ATP and luciferin on firefly luciferase activity.

ATP and luciferin are not only substrates of firefly luciferase, but can, in addition, modulate its activity. High concentrations of luciferin induce a conformational change of the enzyme that temporarily reduces the catalytic rate. Re-activation takes approx. 20 min and is independent of variation in the concentration of enzyme or ATP, but lengthens with increasing luciferin concentration. Hig...

Determination of porcine blood creatine kinase by firefly luciferase.

The use of the creatine kinase-luciferase (CK-L) coupled reaction for porcine blood CK determination was studied. Adenosine triphosphate produced by CK is reacted with firefly L light-producing system. The method permits determination of CK in blood as opposed to traditional methods which require plasma or serum. The luciferase procedure was applied to nine barrows each of purebred Pietrain and...

Detection of local ATP release from activated platelets using cell surface-attached firefly luciferase.

We have developed a method for measuring the local concentration of ATP at the extracellular surface of live cells. This method relies on the specific attachment to the cell surface of a chimeric protein that consists of the IgG-binding domain of Staphylococcus aureus protein A fused in-frame with the complete sequence for firefly luciferase (proA-luc). Expression of proA-luc in Escherichia col...